In vitro assays

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At Methodex we develop in vitro assays. Our specialists have extensive experience in conducting in vitro tests

• Genotoxicity tests:
  •     AMES test (OECD 471).
  •     Micronuclide assay (MNT) (OECD 487).
  •     Comet assay (ASTM E2 186-02A).
  •     Genetic mutation in mammalian cells (MLA) (OECD 476).
     
• Assajos of experimental toxicology in vitro:
  •     In vitro dermal corrosion test (RHE) (OECD 431).
  •     Skin irritation test (OECD 435) (Skinethic RHE).
  •     Phototoxicity assay in Balb / C or 3T3 cells (OECD 432) or with RHE.
  •     Local lymphatic nodule test (OECD 429).
  •     Sensitization test in human THP-1 cells (CD86 / CD54) (ECVAM).
  •     Dermal absorption in reconstituted human skin or pig skin (OECD 428).
  •     EpiOcular (test of ocular irritation in vitro, SIRC-NRU) or HET-CAM (ECVAM).
  •     Systematic cytotoxicity assay (keratinocytes, fibroblasts, B16, ...).
  •     MTT (INVITTOX 17).
  •     Neutral network (INVITTOX 64).
  •     Cell Transformation Assay in 3T3 A31.1-1 (CTA) (ENV / JM / MONO (2016) 1).
  •     Hemolysis test by surfactants (RBC) (INVITTOX 37).